Active Recombinant Human FGF10 protein, His-Avi-tagged, Biotinylated

• Cat.No.: FGF10-1428H
• Product Overview: Biotinylated Recombinant Human FGF10 protein(Gln38-Ser208), fused with His and Avi tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 1 mM TCEP, 0.5 M Arginine, pH 7.4 with trehalose as protectant.
• Bio-activity: Biotinylated Human FGF-10, His,Avitag immobilized on SA Chip can bind Human FGFR2 (IIIb), His Tag with an affinity constant of 5.02 μM as determined in a SPR assay (Biacore 8K).
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 22.9 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Gln38-Ser208
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 38-208 a.a.

Gene Information

• Gene Name: FGF10 fibroblast growth factor 10 [ Homo sapiens ]
• Official Symbol: FGF10
• Synonyms: FGF10; fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• Gene ID: 2255
• mRNA Refseq: NM_004465
• Protein Refseq: NP_004456
• MIM: 602115
• UniProt ID: O15520