Active Recombinant Human FCGR2B Protein, His & Avi-tagged, Biotinylated

• Cat.No.: FCGR2B-324H
• Product Overview: Biotinylated Recombinant Human FCGR2B Protein(Ala 46-Ile 224) (NP_001002274.1) was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Lyophilized from sterile PBS, pH 7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human IgG1 in functional ELISA.
  2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated bound to Avastin was 0.02 μM.
• Molecular Mass: The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation.
• Protein length: Ala 46-Ile 224
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR2B Fc fragment of IgG, low affinity IIb, receptor (CD32) [ Homo sapiens ]
• Official Symbol: FCGR2B
• Synonyms: FCGR2B; CD32; CD32B; CDw32; fcRII-b; Fc gamma RIIb; FCG2; FCGR2; IGFR2
• Gene ID: 2213
• mRNA Refseq: NM_001002273.2
• Protein Refseq: NP_001002273.1
• MIM: 604590
• UniProt ID: P31994