Active Recombinant Human FCGR1A(Gln 16 - Pro 288), His-tagged, Biotinylated

• Cat.No.: FCGR1A-180H
• Product Overview: Biotinylated Human Fc gamma RI / CD64 Protein is expressed from human HEK293 cells. It contains AA Gln 16 - Pro 288 (Accession # AAH32634).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 16-288 a.a.
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/mL.
• Molecular Mass: This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 34.3 kDa. The predicted N-terminus is Gln 16. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FCGR1A Fc fragment of IgG, high affinity Ia, receptor (CD64) [ Homo sapiens ]
• Official Symbol: FCGR1A
• Synonyms: FCGR1A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; CD64; CD64A; fcgammaRIa; Fc-gamma RI; fc-gamma RIA; Fc gamma receptor; IgG Fc receptor I; Fc-gamma receptor I A1; FCRI; IGFR1; FLJ18345
• Gene ID: 2209
• mRNA Refseq: NM_000566
• Protein Refseq: NP_000557
• MIM: 146760
• UniProt ID: P12314