Active Recombinant Human FAS
Cat.No. : | FAS-519H |
Product Overview : | Recombinant Human FAS produced in E. coli is approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. |
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Species : | Human |
Source : | E.coli |
Tag : | Non |
Description : | Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. |
Form : | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 determined by its ability to inhibit the cytotoxicity of Jurkat cells is less than 15 μg/ml, corresponding to a specific activity of >66.7 IU/mg, in the presence of 2ng/ml of rHuFas Ligand. |
Molecular Mass : | 17.6 kDa |
AA Sequence : | RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN |
Endotoxin : | Less than 1EU/μg of rHusFasR/TNFRSF6 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analyses. |
Usage : | This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Storage : | This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Reconstitution : | We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name | FAS Fas (TNF receptor superfamily, member 6) [ Homo sapiens ] |
Official Symbol | FAS |
Synonyms | FAS; Fas (TNF receptor superfamily, member 6); APT1, FAS1, TNFRSF6, tumor necrosis factor receptor superfamily, member 6; tumor necrosis factor receptor superfamily member 6; APO 1; CD95; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; apoptosis-mediating surface antigen FAS; tumor necrosis factor receptor superfamily, member 6; APT1; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; |
Gene ID | 355 |
mRNA Refseq | NM_000043 |
Protein Refseq | NP_000034 |
MIM | 134637 |
UniProt ID | P25445 |
Chromosome Location | 10q24.1 |
Pathway | Activation of Pro-Caspase 8, organism-specific biosystem; Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Alzheimers disease, organism-specific biosystem; |
Function | binding; identical protein binding; kinase binding; protein binding; receptor activity; receptor activity; signal transducer activity; transmembrane signaling receptor activity; tumor necrosis factor-activated receptor activity; |
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Not For Human Consumption!
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