Active Recombinant Human FAP protein, Fc-tagged

• Cat.No.: FAP-1446H
• Product Overview: Recombinant Human FAP protein(Leu26-Asp760), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 26-760 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Human FAP, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.2-3 ng/mL.Immobilized Anti-FAP Antibody, Mouse IgG2a (FAP5) at 1 μg/mL (100 μL/well) can bind Human FAP, Fc Tag with a linear range of 0.5-4 ng/mL.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >7000 pmol/min/µg.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 111.5 kDa. The protein migrates as 110-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu26-Asp760
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FAP fibroblast activation protein, alpha [ Homo sapiens ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451
• MIM: 600403
• UniProt ID: Q12884