Active Recombinant Human F10 Protein, His-tagged

• Cat.No.: F10-1105H
• Product Overview: Recombinant Human F10 (Accession # P00742) Met1-Lys488, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Phe80, Tyr84, & Ile235
• Form: Lyophilized from a 0.2 µm filtered solution in MES, NaCl and CaCl2.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >800 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Recombinant Human F10, His-tagged has a calculated MW of 16.6 kDa & 30 kDa. In SDS-PAGE migrates as 12-15 kDa and 30-36 kDa, reducing conditions.
• Protein length: Met1-Lys488
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: F10 coagulation factor X [ Homo sapiens ]
• Official Symbol: F10
• Synonyms: F10; coagulation factor X; factor Xa; prothrombinase; Stuart-Prower factor; FX; FXA
• Gene ID: 2159
• mRNA Refseq: NM_000504
• Protein Refseq: NP_000495
• MIM: 613872
• UniProt ID: P00742
• Chromosome Location: 13q34