Active Recombinant Human ERBB3 protein(Pro 730-Ser 1065), His&GST-tagged

• Cat.No.: ERBB3-7276H
• Product Overview: Active Recombinant Human ERBB3 protein(NP_001973.2)(Pro730-Ser1065), fused with N-terminal His and GST tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Pro 730-Ser 1065
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 1mM GSH.
• Bio-activity: 1. No Kinase Activity. 2. Using the Octet RED System, the affinity constant (Kd) of human ErBB3-GST bound to human NRG1 (aa 2-224) was 20 nM.
• Molecular Mass: The recombinant human ErbB3 /GST chimera consists of 573 amino acids and has a calculated molecular mass of 65 KDa. It migrates as an approximately 65 KDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 75 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB3 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) [ Homo sapiens ]
• Official Symbol: ERBB3
• Synonyms: ERBB3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian); LCCS2, lethal congenital contracture syndrome 2; receptor tyrosine-protein kinase erbB-3; HER3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; MGC88033
• Gene ID: 2065
• mRNA Refseq: NM_001005915
• Protein Refseq: NP_001005915
• MIM: 190151
• UniProt ID: P21860