Active Recombinant Human ERBB2 Protein, DDDDK-tagged, Alexa Fluor 555 conjugated

• Cat.No.: ERBB2-177HAF555
• Product Overview: A DNA sequence encoding the human ERBB2 (NP_004439.2) (Met1-Thr652) (Alexa Fluor 555 conjugated) was expressed with five amino acids (DDDDK) at the C-terminus inHEK293 cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 1-652 a.a.
• Description: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Human Her2/ERBB2 at 2 μg/mL (100 μL/well) can bind Herceptin, the EC50 of Herceptin is 7.0-30.0 ng/mL.
  2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.4-2.4 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
• Molecular Mass: 70 kDa
• N-terminal Sequence Analysis: Thr 23
• Endotoxin: < 1.0 EU/ μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
  Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
• Shipping: At ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Gene ID: 2064
• mRNA Refseq: NM_004448
• Protein Refseq: NP_004439
• MIM: 164870
• UniProt ID: P04626

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.