Active Recombinant Human EPHA2 Protein (Gln25-Asn534), C-6×His tagged

• Cat.No.: EPHA2-27H
• Product Overview: Recombinant human EPHA2 protein (Gln25-Asn534) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: His
• Protein Length: Gln25-Asn534
• Description: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rhEphA2 at 2 μg/mL (100 μL/well) can bind rmEphrin-A1/Fc Chimera with a linear range of 0.078-5 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 56.9 kDa
  SDS-PAGE: 70-75 kDa, reducing conditions
• N-terminal Sequence Analysis: No results obtained: Gln25 predicted & Lys27
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: EPHA2 EPH receptor A2 [ Homo sapiens (human) ]
• Official Symbol: EPHA2
• Synonyms: EPHA2; EPH receptor A2; ECK, EphA2; ephrin type-A receptor 2; soluble EPHA2 variant 1; tyrosine-protein kinase receptor ECK; epithelial cell receptor protein tyrosine kinase; ECK; CTPA; ARCC2; CTPP1
• Gene ID: 1969
• mRNA Refseq: NM_004431
• Protein Refseq: NP_004422
• MIM: 176946
• UniProt ID: P29317