Active Recombinant Human EphA10 protein, His-tagged

• Cat.No.: EphA10-1316H
• Product Overview: Recombinant Human EphA10 protein(Pro 27 - Pro 564), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro27-Pro564
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human EphA10, His Tag at 5 μg/mL (100 μL/well) can bind Human Ephrin-A3, Fc Tag with a linear range of 0.1-2 ng/mL.Immobilized Human Ephrin-A3, Fc Tag at 5 μg/mL (100 μL/well) can bind Human EphA10, His Tag with a linear range of 0.005-0.313 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 60.2 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA10 EPH receptor A10 [ Homo sapiens ]
• Official Symbol: EphA10
• Synonyms: EPHA10; EPH receptor A10; ephrin type-A receptor 10; FLJ16103; FLJ33655; EphA10s protein; MGC43817
• Gene ID: 284656
• mRNA Refseq: NM_001099439
• Protein Refseq: NP_001092909
• MIM: 611123
• UniProt ID: Q5JZY3