Active Recombinant Human EFEMP1 protein, HA-tagged

• Cat.No.: EFEMP1-68H
• Product Overview: Recombinant Human EFEMP1(Gln18-Phe493) fused with HA tag at N-terminal was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: HA
• Predicted N Terminal: Tyr (of HA tag)
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is typically 0.4-2 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 54 kDa;
  SDS-PAGE: 52-71 kDa, reducing conditions.
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Protein length: 18-493 a.a.

Gene Information

• Gene Name: EFEMP1 EGF containing fibulin-like extracellular matrix protein 1 [ Homo sapiens ]
• Official Symbol: EFEMP1
• Synonyms: EFEMP1; EGF containing fibulin-like extracellular matrix protein 1; DHRD, EGF containing fibulin like extracellular matrix protein 1 , FBNL, fibrillin like; EGF-containing fibulin-like extracellular matrix protein 1; FBLN3; fibulin 3; MTLV; S1 5; FIBL-3; fibulin-3; extracellular protein S1-5; DHRD; DRAD; FBNL; MLVT; S1-5; FLJ35535; MGC111353
• Gene ID: 2202
• mRNA Refseq: NM_001039348
• Protein Refseq: NP_001034437
• MIM: 601548
• UniProt ID: Q12805
• Chromosome Location: 2p16
• Function: calcium ion binding; epidermal growth factor receptor binding; epidermal growth factor-activated receptor activity; growth factor activity; protein binding