Active Recombinant Human DSG2 protein, His-tagged

• Cat.No.: DSG2-510H
• Product Overview: Recombinant Human DSG2 protein(Q14126)(Ala50-Gly609) is expressed from HEK293 with His tag at the C-terminal.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DSG-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DSG-2 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
• Protein length: Ala50-Gly609
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DSG2 desmoglein 2 [ Homo sapiens ]
• Official Symbol: DSG2
• Synonyms: DSG2; desmoglein 2; desmoglein-2; CDHF5; HDGC; cadherin family member 5; ARVC10; ARVD10; CMD1BB; MGC117034; MGC117036; MGC117037
• Gene ID: 1829
• mRNA Refseq: NM_001943
• Protein Refseq: NP_001934
• MIM: 125671
• UniProt ID: Q14126