Active Recombinant Human DLL4 protein, His-tagged

• Cat.No.: DLL4-2235H
• Product Overview: Recombinant Human DLL4 protein(NP_061947.1)(Met1-Pro524), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Pro524
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human Notch1 in a functional ELISA.2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
• Molecular Mass: The recombinant human DLL4 consists of 509 amino acids and predicts a molecular mass of 55.7 kDa. As a result of glycosylation, the apparent molecular mass of rhDLL4 is approximately 55-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DLL4 delta-like 4 (Drosophila) [ Homo sapiens ]
• Official Symbol: DLL4
• Synonyms: DLL4; delta-like 4 (Drosophila); delta like 4 homolog (Drosophila); delta-like protein 4; delta4; delta 4; delta ligand 4; notch ligand DLL4; delta-like 4 homolog; delta-like 4 protein; notch ligand delta-2; drosophila Delta homolog 4; hdelta2; MGC126344
• Gene ID: 54567
• mRNA Refseq: NM_019074
• Protein Refseq: NP_061947
• MIM: 605185
• UniProt ID: Q9NR61