Active Recombinant Human DKK1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: DKK1-278H
• Product Overview: Biotinylated Recombinant Human DKK1 protein(Thr 32 - His 266), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human Dkk-1, Fc,Avitag at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human LRP-5, Mouse IgG2a Fc Tag with a linear range of 0.5-16 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Thr32-His266
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 32-266 a.a.

Gene Information

• Gene Name: DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: DKK1
• Synonyms: DKK1; dickkopf 1 homolog (Xenopus laevis); dickkopf (Xenopus laevis) homolog 1; dickkopf-related protein 1; DKK 1; SK; hDkk-1; dickkopf-1 like; dickkopf related protein-1; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242
• Protein Refseq: NP_036374
• MIM: 605189
• UniProt ID: O94907