Active Recombinant Human DDAH1 Protein, Met & His-tagged

• Cat.No.: DDAH1-1063H
• Product Overview: Recombinant Human DDAH1 (Accession # O94760) Ala2-Ser285, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Inconclusive result, Met predicted. Protein identity confirmed by MS analysis of tryptic fragments.
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol and DTT.
• Bio-activity: Measured by its ability to hydrolyze asymmetric dimethylarginine to L-citrulline and dimethylamine.The specific activity is >75 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human DDAH1, Met & His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 38 kDa, reducing conditions.
• Protein length: Ala2-Ser285
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 100 mM acetic acid containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: DDAH1 dimethylarginine dimethylaminohydrolase 1 [ Homo sapiens ]
• Official Symbol: DDAH1
• Synonyms: DDAH1; dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; DDAH-1; dimethylargininase-1; NG, NG-dimethylarginine dimethylaminohydrolase; FLJ21264; FLJ25539
• Gene ID: 23576
• mRNA Refseq: NM_001134445
• Protein Refseq: NP_001127917
• MIM: 604743
• UniProt ID: O94760
• Chromosome Location: 1p22