Active Recombinant Human CYP3A7 Protein

• Cat.No.: CYP3A7-05H
• Product Overview: Recombinant human CYP3A7 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene.
• Bio-activity: Cytochrome c Reductase Activity: 667 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Testosterone 6β-hydroxylase
  Linear with time up to: 8 min(Determined at 32 µM substrate, using a P450 concentration of 50 pmol/ml)
  Linear with P450 up to: 60 pmol/ml(Determined at 32 µM substrate, using an incubation time of 10 min)
  Vmax: 2.2 pmol/min/pmol CYP
  Km: 13 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP3A7 cytochrome P450 family 3 subfamily A member 7 [ Homo sapiens (human) ]
• Official Symbol: CYP3A7
• Synonyms: CYP3A7; cytochrome P450 family 3 subfamily A member 7; CP37; CYPIIIA7; P450HLp2; P450-HFLA; P-450111A7; P-450(HFL33); cytochrome P450 3A7; aryl hydrocarbon hydroxylase; cytochrome P450, family 3, subfamily A, polypeptide 7; cytochrome P450, subfamily IIIA, polypeptide 7; cytochrome P450-HFLA; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.14.1
• Gene ID: 1551
• mRNA Refseq: NM_000765
• Protein Refseq: NP_000756
• MIM: 605340
• UniProt ID: P24462