Active Recombinant Human CYP1A1 Protein

Description :	This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids.
Source :	E.coli
Species :	Human
Form :	50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Bio-activity :	Specific P450 Content:100 pmol/mg protein; Cytochrome c Reductase Activity:103 nmol/min/mg protein; P450 Activity:7-ethoxycoumarin O-deethylase; Linear with time up to:12 min; Linear with P450 up to:5 pmol/ml; Vmax:11 pmol/min/pmol CYP; Km:3.8 μM
Notes :	1. Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]; 2. Determined at 5 μM substrate, using a P450 concentration of 1 pmol/ml; 3. Determined at 5 μM substrate, using an incubation time of 5 min.
Storage :	Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration :	10 mg/ml
Tag :	Non