Active Recombinant Human CXCL4 protein, His-tagged

• Cat.No.: CXCL4-1295H
• Product Overview: Recombinant Human CXCL4 protein( Glu 32 - Ser 101), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Glu 32 - Ser 101
• Form: Lyophilized from 0.22 μm filtered solution in 10 mM Sodium Citrate,150 mM NaCl,pH5.0 with trehalose as protectant.
• Bio-activity: Immobilized Human CXCL4, His Tag at 5 μg/mL (100 μL/well) can bind Human CCL5, Fc Tag with a linear range of 0.078-1.25 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 9.7 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.