Active Recombinant Human CTRC protein, His-tagged

• Cat.No.: CTRC-2508H
• Product Overview: Recombinant Human CTRC protein(Q99895)(Met1-Leu268), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu268
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, SUC­Ala­Ala­Pro­Phe­AMC. The specific activity is >300 pmol/min/μg.(Activation of this protease is needed prior to the use in activity assays)
• Molecular Mass: The secreted recombinant human CTRC (pro form) consists of 263 amino acids and has a predicted molecular mass of 29.3 kDa. It migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTRC chymotrypsin C (caldecrin) [ Homo sapiens ]
• Official Symbol: CTRC
• Synonyms: CTRC; chymotrypsin C (caldecrin); chymotrypsin-C; CLCR; ELA4; elastase 4; caldecrin; elastase IV; serum calcium decreasing factor
• Gene ID: 11330
• mRNA Refseq: NM_007272
• Protein Refseq: NP_009203
• MIM: 601405
• UniProt ID: Q99895