Active Recombinant Human CSNK2A2

• Cat.No.: CSNK2A2-169H

Specification

• Cat. No.: CSNK2A2-169H
• Description: Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. The enzyme is probably present in all eukaryotic cells, implying that it has fundamental cellular functions. Similar to protein kinase A, CK2 is a tetramer containing 2 α-prime subunits (or one of each) and 2β-subunits. The subunit fills a regulatory role in the holoenzyme. The catalytic a-subunit corresponds to the C-subunit of PKA, the non-catalytic-subunit is unique and differs from the Rsubunit of PKA in all known features. The α-prime subunits are the catalytic subunits with distinct sequences and are encoded by different genes. Whereas the CK2 α-subunit is found ubiquitously in all cells and organs the CK2 α-prime subunit is preferentially found in brain and testis. Recently it was shown that theα-subunit is trifunctional: (i) it confers stability to the holoenzyme, (ii) it increases enzyme activity, and (iii) it determines substrate specificity. Disruption of the CK2 alpha prime gene in mice results in oligospermia and globozoospermia. Thus, the CK2 alpha prime gene may be a candidate gene for inherited abnormalities of sperm morphogenesis The human recombinant protein kinase CK2 was expressed in E. coli as a N-MBP fusion protein.
• Source: E. coli.
• Usage: For in vitro use only.
• Form: Supplied in 20 mM Tris-HCl pH 7.4, 200 mM NaCl, 1 mM EDTA, and 2 mM DTT.
• Activity: > 100.000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide RRRDDDSDDD per min at 37℃).
• Purity: > 99% by SDS-PAGE.
• Storage: Quality guaranteed for 12 months, Store at -80℃. Avoid freeze / thaw cycles.
• Tag: Non

Gene Information

• Gene Name: CSNK2A2 casein kinase 2, alpha prime polypeptide [ Homo sapiens ]
• Synonyms: CSNK2A2; casein kinase 2, alpha prime polypeptide; CK2A2; CSNK2A1; FLJ43934; EC 2.7.11.1; CK II; Casein kinase II subunit alpha
• Gene ID: 1459
• mRNA Refseq: NM_001896
• Protein Refseq: NP_001887
• MIM: 115442
• UniProt ID: P19784
• Chromosome Location: 16
• Pathway: Adherens junction; Tight junction; Wnt signaling pathway
• Function: ATP binding; nucleotide binding; protein N-terminus binding; protein serine/threonine kinase activity; transferase activity