Active Recombinant Human CSF1 Protein, His-tagged

• Cat.No.: CSF1-426H
• Product Overview: Recombinant human CSF1 (NP_757349.1) (Glu33-Asn190) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: Yeast
• Species: Human
• Tag: His
• Predicted N Terminal: Glu 33
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using M?NFS?60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1-4 ng/mL.
• Molecular Mass: The recombinant human CSF1 consists 168 amino acids and predicts a molecular mass of 19.8 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >(45.8+27.8+25.7) % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 33-190 a.a.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• UniProt ID: P09603
• Chromosome Location: 1p21-p13
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Integrins in angiogenesis, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem