Active Recombinant Human CNTN5 protein, His-tagged

Cat.No. : CNTN5-673H
Product Overview : Recombinant Human CNTN5 protein(NP_055176.1)(Met1-Gln1059), fused with His tag, was expressed in HEK293.
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Species : Human
Source : HEK293
Tag : His
Protein Length : Met1-Gln1059
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN5 coated plates (12.5μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37°C
Molecular Mass : The secreted recombinant human CNTN5 comprises 1052 amino acids with a predicted molecular mass of 116 kDa. As a result of glycosylation, rhCNTN5 migrates as an approximately 130-140 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name CNTN5 contactin 5 [ Homo sapiens ]
Official Symbol CNTN5
Synonyms CNTN5; contactin 5; contactin-5; hNB 2; NB 2; neural adhesion molecule; neural recognition molecule NB-2; NB-2; HNB-2s; MGC163491;
Gene ID 53942
mRNA Refseq NM_001243270
Protein Refseq NP_001230199
MIM 607219
UniProt ID O94779

Not For Human Consumption!

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