Active Recombinant Human CMA1 protein, His-tagged

• Cat.No.: CMA1-25H
• Product Overview: Recombinant Human CMA1(Met1-Asn247) fused with His tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: Met1-Asn247
• Description: Chymases are a group of chymotrypsin-like serine proteases secreted by mast cells. They are synthesized as inactive precursors containing a 2-residue propeptide, which needs to be removed by dipeptidyl peptidase I/cathepsin C for the enzymatic activity. Human Chymase encoded by the CMA1 gene is known to be involved in hypertention and heart failure through its ability to convert angiotensin I (Ang I) to angiotensin II (Ang II), which plays a key role in the regulation of arterial pressure. In addition, it is also important in physiological and pathological conditions including inflammation, fibrosis and processing of cytokines. Therefore, designing a specific inhibitor for Chymase activity has been a pharmacologic strategy to develop therapeutic agents.
• Predicted N Terminal: Gly20
• Form: Supplied as a 0.2 μm filtered solution in HEPES and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC. The specific activity is >80 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 27 kDa;
  SDS-PAGE: 38 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CMA1 chymase 1, mast cell [ Homo sapiens ]
• Official Symbol: CMA1
• Synonyms: CMA1; chymase 1, mast cell; chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; CYH; MCT1; MGC119890; MGC119891
• Gene ID: 1215
• mRNA Refseq: NM_001836
• Protein Refseq: NP_001827
• MIM: 118938
• UniProt ID: P23946
• Chromosome Location: 14
• Pathway: ACE Inhibitor Pathway, organism-specific biosystem; Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Renin-angiotensin system, organism-specific biosystem; Renin-angiotensin system, conserved biosystem; Signal Transduction, organism-specific biosystem
• Function: peptidase activity; serine-type endopeptidase activity; serine-type endopeptidase activity; serine-type peptidase activity