Active Recombinant Human CFD protein, His-Avi-tagged, Biotinylated

• Cat.No.: CFD-3030H
• Product Overview: Biotinylated Recombinant Human CFD protein(Gln 19-Pro 224), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gln 19-Pro 224
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 1 M Nacl, pH8.0 with trehalose as protectant.
• Bio-activity: Immobilized Human CD32a (H167), His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive Protein, His,Avitag with a linear range of 0.02-1.25 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)The protein has a calculated MW of 26.6 kDa. The protein migrates as 29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CFD complement factor D (adipsin) [ Homo sapiens ]
• Official Symbol: CFD
• Synonyms: CFD; complement factor D (adipsin); D component of complement (adipsin) , DF, PFD, properdin factor D; complement factor D; ADN; properdin factor D; C3 convertase activator; complement factor D preproprotein; D component of complement (adipsin); DF; PFD; ADIPSIN
• Gene ID: 1675
• mRNA Refseq: NM_001928
• Protein Refseq: NP_001919
• MIM: 134350
• UniProt ID: P00746