Active Recombinant Human CEACAM5 protein(Lys35-Ala685), His-tagged

• Cat.No.: CEACAM5-3200H
• Product Overview: Active Recombinant Human CEACAM5 protein(P06731-1)(Lys35-Ala685) is expressed from HEK293 with His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Lys35-Ala685
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CEACAM-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 72.42 kDa. Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ]
• Official Symbol: CEACAM5
• Synonyms: CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; DKFZp781M2392
• Gene ID: 1048
• mRNA Refseq: NM_004363
• Protein Refseq: NP_004354
• MIM: 114890
• UniProt ID: P06731