Active Recombinant Human CDNF

• Cat.No.: CDNF-537H
• Product Overview: Recombinant Human CDNF produced in E. coli is approximately 18.3 kDa, a single non-glycosylated polypeptide chain containing 161 amino acids.

Specification

• Description: Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is encoded by the CDNF gene in humans and widely expressed in neuronal and non-neuronal tissues. The CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). Furthermore, it also prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. By research, CDNF might be beneficial for the treatment of Parkinson"s disease. Recombinant human CDNF contains 162 amino acid residues and it shares 81 % and 84 % a.a. sequence identity with murine and rat CDNF.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 150 mM NaCl, pH 6.4, 3% trehalose.
• Bio-activity: Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using rat C6 cells is less than 25 μg/ml, corresponding to a specific activity of >4 × 10^4 IU/mg.
• Molecular Mass: 18.3 kDa
• AA Sequence: QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L
• Endotoxin: Less than 1 EU/μg of rHuCDNF as determined by LAL method.
• Purity: >96% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: CDNF cerebral dopamine neurotrophic factor [ Homo sapiens ]
• Official Symbol: CDNF
• Synonyms: CDNF; cerebral dopamine neurotrophic factor; arginine rich, mutated in early stage tumors like 1 , ARMETL1; conserved dopamine neurotrophic factor; ARMET-like protein 1; arginine-rich, mutated in early stage tumors-like 1; ARMETL1
• Gene ID: 441549
• mRNA Refseq: NM_001029954
• Protein Refseq: NP_001025125
• MIM: 611233
• UniProt ID: Q49AH0
• Chromosome Location: 10p13
• Function: growth factor activity