Active Recombinant Human CDH3 protein, His/Fc-tagged

• Cat.No.: CDH3-488H
• Product Overview: Recombinant Human CDH3(Asp108 - Gly654) fused with His/Fc tag at C-terminal was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc&His
• Protein Length: 108-654 a.a.
• Predicted N Terminal: Asp108
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 10^5 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.Optimal concentration depends on cell type as well as the application or research objectives.
• Molecular Mass: Predicted Molecular Mass: 87.4 kDa;SDS-PAGE: 120-130 kDa, under reducing conditions.
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: CDH3 cadherin 3, type 1, P-cadherin (placental) [ Homo sapiens ]
• Official Symbol: CDH3
• Synonyms: CDH3; cadherin 3, type 1, P-cadherin (placental); cadherin 3, P cadherin (placental); cadherin-3; CDHP; PCAD; calcium-dependent adhesion protein, placental; HJMD
• Gene ID: 1001
• mRNA Refseq: NM_001793
• Protein Refseq: NP_001784
• MIM: 114021
• UniProt ID: P22223
• Chromosome Location: 16q22.1
• Pathway: Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem
• Function: calcium ion binding