Active Recombinant Human CDH16 protein, His-tagged

• Cat.No.: CDH16-3860H
• Product Overview: Recombinant Human CDH16 protein(NP_004053.1)(Met1-Ala786), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ala786
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to CDH16-His coated plates (10 μg/mL, 100 μL/well), approximately 20-40% will adhere specifically after 60 minutes at 37 °C.
• Molecular Mass: The recombinant human CDH16 comprises 779 amino acids and has a calculated molecular mass of 84.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CDH16 cadherin 16, KSP-cadherin [ Homo sapiens ]
• Official Symbol: CDH16
• Synonyms: CDH16; cadherin 16, KSP-cadherin; cadherin-16; KSP-cadherin; kidney-specific cadherin
• Gene ID: 1014
• mRNA Refseq: NM_001204744
• Protein Refseq: NP_001191673
• MIM: 603118
• UniProt ID: O75309