Active Recombinant Human CDC42 protein, GST-tagged

• Cat.No.: CDC42-2635H
• Product Overview: Recombinant Human CDC42 fused with GST tag in the amino-terminus was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
• Form: 2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose, 0.1% dextran.
• Bio-activity: Biological activity of the protein is determined by demonstrating that in GTP bound CDG01 associates with PAK protein while the GDP bound form of CDG01 does not associate with PAK protein in a pull down assay.
• Molecular Mass: ~50 kDa
• Purity: >90% pure
• Applications: Cdc42 biochemistry; Cdc42 GTPase assays; Cdc42 nucleotide exchange assays; Cdc42 binding studies
• Reconstitution: reconstituted in distilled water to 1 mg/ml

Gene Information

• Gene Name: CDC42 cell division cycle 42 (GTP binding protein, 25kDa) [ Homo sapiens ]
• Official Symbol: CDC42
• Synonyms: CDC42; cell division cycle 42 (GTP binding protein, 25kDa); cell division cycle 42 (GTP binding protein, 25kD); cell division control protein 42 homolog; CDC42Hs; G25K; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
• Gene ID: 998
• mRNA Refseq: NM_001039802
• Protein Refseq: NP_001034891
• MIM: 116952
• UniProt ID: P60953
• Chromosome Location: 1p36.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Axon guidance, organism-specific biosystem
• Function: GTP binding; GTP-dependent protein binding; GTPase activity; GTPase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; thioesterase binding