Active Recombinant Human CD86 protein, Fc-tagged

• Cat.No.: CD86-2903H
• Product Overview: Recombinant Human CD86 protein(Leu 26-Pro 247), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human B7-2, Fc Tag, premium grade at 5 μg/mL (100 μL/well) can bind Biotinylated Human CTLA-4, Fc,Avitag with a linear range of 0.6-10 ng/mL.Immobilized Biotinylated Mouse CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human B7-2, Fc Tag, premium grade with a linear range of 5-78 ng/mL.Immobilized Biotinylated Human / Cynomolgus / Rhesus macaque CD28, His,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human B7-2, Fc Tag, premium grade with a linear range of 2-78 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.5 kDa. The protein migrates as 66-100 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Protein length: Leu 26-Pro 247
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD86 CD86 molecule [ Homo sapiens ]
• Official Symbol: CD86
• Synonyms: CD86; CD86 molecule; CD28LG2, CD86 antigen (CD28 antigen ligand 2, B7 2 antigen); T-lymphocyte activation antigen CD86; B lymphocyte antigen B7 2; B7 2; B7.2; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B70; B7-2; LAB72; CD28LG2; MGC34413
• Gene ID: 942
• mRNA Refseq: NM_001206924
• Protein Refseq: NP_001193853
• MIM: 601020
• UniProt ID: P42081