Active Recombinant Human CD86 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD86-585H
• Product Overview: Biotinylated Recombinant Human CD86 Protein (Leu 26 - Pro 247) (AAH40261), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Leu 26 - Pro 247
• Predicted N Terminal: Leu 26
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc Tag with a linear range of 19-156 ng/mL.
• Molecular Mass: Recombinant Human CD86 Protein has a calculated MW of 53.8 kDa. As a result of glycosylation, The protein migrates as 66-100 kDa under reducing (R) condition and 120-140 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD86 CD86 molecule [ Homo sapiens ]
• Official Symbol: CD86
• Synonyms: CD86; CD86 molecule; CD28LG2, CD86 antigen (CD28 antigen ligand 2, B7 2 antigen); T-lymphocyte activation antigen CD86; B lymphocyte antigen B7 2; B7 2; B7.2; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); B70; B7-2; LAB72; CD28LG2; MGC34413
• Gene ID: 942
• mRNA Refseq: NM_001206924
• Protein Refseq: NP_001193853
• MIM: 601020
• UniProt ID: P42081
• Chromosome Location: 3q21
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem
• Function: coreceptor activity; protein binding; receptor activity