Active Recombinant Human CD59, His-tagged

• Cat.No.: CD59-1600H
• Product Overview: Recombinant Human CD59 (Leu26-Asn102) fused with a C­terminal 10­His tag, was expressed in Mouse NSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 26-102 a.a.
• Description: This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
• Molecular Mass: 10.3 kDa
• Predicted N terminal: Leu26
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its binding ability in a functional ELISA. When recombinant human C9 is coated at 5 µg/mL (100 µL/well), the concentration of Recombinant Human CD59 that produces 50% optimal binding response is typically 0.6‑3 µg/mL.
• EndotoxinLevel: < 0.01 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 µg/mL in PBS.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: CD59

Gene Information

• Gene Name: CD59 CD59 molecule, complement regulatory protein [ Homo sapiens ]
• Synonyms: CD59; CD59 molecule, complement regulatory protein; CD59 antigen p18 20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) , CD59 antigen, complement regulatory protein , MIC11, MIN1, MIN2, MIN3, MSK21; CD59 glycoprotein; 16.3A5; EJ16; EJ30; EL32; G344; p18 20; protectin; 1F5 antigen; MEM43 antigen; Ly-6-like protein; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; 20 kDa homologous restriction factor; membrane inhibitor of reactive lysis; membrane attack complex inhibition factor; membrane attack complex (MAC) inhibition factor; surface anitgen recognized by monoclonal 16.3A5; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); 1F5; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; HRF-20; MAC-IP; p18-20; MGC2354; FLJ38134; FLJ92039
• Gene ID: 966
• mRNA Refseq: NM_000611
• Protein Refseq: NP_000602
• MIM: 107271
• UniProt ID: P13987
• Chromosome Location: 11p13
• Pathway: Arf6 trafficking events; Complement and coagulation cascades; Hematopoietic cell lineage
• Function: protein binding; complement binding