Active Recombinant Human CD58 protein, hFc-tagged

• Cat.No.: CD58-543H
• Product Overview: Recombinant Human CD58 protein(Q9BRW0)(Met1-Arg215), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg215
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His (Cat:10982-H08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His (Cat:90300-C08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 μg/ml.
• Molecular Mass: The recombinant human CD58/Fc is a disulfide-linked homodimer. The reduced monomer comprises 428 amino acids and has a predicted molecular mass of 48.5 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD58 CD58 molecule [ Homo sapiens ]
• Official Symbol: CD58
• Synonyms: CD58; CD58 molecule; CD58 antigen, (lymphocyte function associated antigen 3) , LFA3; lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3); ag3; LFA3; LFA-3; FLJ23181; FLJ43722
• Gene ID: 965
• mRNA Refseq: NM_001144822
• Protein Refseq: NP_001138294
• MIM: 153420
• UniProt ID: P19256