Active Recombinant Human CD30 protein, Fc-tagged,FITC-Labeled

• Cat.No.: CD30-3074H
• Product Overview: FITC-Labeled Recombinant Human CD30 protein(Phe 19-Lys 379), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Phe 19-Lys 379
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monoclonal Anti-Human CD30 Human Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human CD30, Fc Tag with a linear range of 0.08-1.3 μg/mL.Immobilized Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human CD30, Fc Tag with a linear range of 0.2-1.3 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 64.6 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.