Active Recombinant Human CD274 Protein, MIgG2a mFc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD274-002HAF555
• Product Overview: The Alexa Fluor 555 conjugated extracellular domain of human PDL1 (NP_054862.1) (Phe19-Arg238) is fused to the N-terminus of the Fc region of mouse IgG2a was expressed in CHO cell.

Specification

• Species: Human
• Source: CHO
• Tag: mFc
• Protein Length: 19-238 a.a.
• Description: CD274 (CD274 molecule) is a protein-coding gene. Diseases associated with CD274 include pulmonary venoocclusive disease, and htlv-1 associated myelopathy/tropical spastic paraparesis, and among its related super-pathways are Immune System and Phosphorylation of CD3 and TCR zeta chains. GO annotations related to this gene include protein binding
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
• Molecular Mass: 54 kDa (monomer)
• AA Sequence: Phe19-Arg238
• Endotoxin: < 0.06 EU/ μg as determined by LAL test.
• Purity: > 98 % by SDS-PAGE under reducing conditions.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Stable for at least 1 year after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7