Active Recombinant Human CD274 protein, mFc-tagged

• Cat.No.: CD274-1003H
• Product Overview: Recombinant Human CD274 protein(Phe 19 - Arg 238), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Phe 19 - Arg 238
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human PD-1, Strep Tag at 1 μg/mL (100 μL/well) can bind Human PD-L1 Protein, Mouse IgG1 Fc Tag with a linear range of 5-78 ng/mL.Serial dilutions of Nivolumab were added into Human PD-L1 Protein, Mouse IgG1 Fc Tag: Human PD-1, Strep Tag binding reactions. The half maximal inhibitory concentration (IC50) is 0.2391 μg/mL.
• Molecular Mass: This protein carries a mouse IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 51.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7