Active Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated

• Cat.No.: CD274-365H
• Product Overview: Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Predicted N-terminus: Phe 19. This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag is inserted in-between the Fc and his tags. The protein has a calculated MW of 53.2 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc&His
• Protein Length: Phe 19 - Arg 238
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PD-1, Fc Tag at 2 μg/mL (100μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.01-0.25 μg/mL (QC tested).
  Immobilized Human B7-1, mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with a linear range of 0.02-0.625 μg/mL (Routinely tested).
  Flow Cytometry assay shows that recombinant Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated can bind to 293 cell overexpressing human PD-1. The concentration of PD-L1 used is 1 μg/ml (Routinely tested).
  FACS analysis shows that the binding of Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated to 293 overexpressing PD-1 was inhibited by increasing concentration of neutralizing anti-PD-1 antibody. The concentration of PD-L1 used is 2μg/ml. The IC50 is 0.64 μg/ml (Routinely tested).
  Immobilized cell surface PD-1(5x104 of cells per well) can bind Recombinant Human CD274 Protein, His & Fc & Avi-tagged, Biotinylated with an EC50 of 0.082ug/ml (Routinely tested).
• Molecular Mass: 53.2 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD274 CD274 molecule [ Homo sapiens ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 molecule; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296
• Gene ID: 29126
• mRNA Refseq: NM_014143
• Protein Refseq: NP_054862
• MIM: 605402
• UniProt ID: Q9NZQ7