Active Recombinant Human CD244 protein, hFc&Avi-tagged, Biotinylated

• Cat.No.: CD244-2225H
• Product Overview: Biotinylated Recombinant Human CD244 protein(NP_057466.1)(Met1-Arg221), fused with hFc&Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Met1-Arg221
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD48 hFc at 2 μg/mL (100 μL/well) can bind Human 2B4/CD244 (hFc & AVI Tag), Biotinylated, the EC50 of Human 2B4/CD244 (hFc & AVI Tag), Biotinylated is 10-70 ng/mL.
• Molecular Mass: The recombinant human CD244 consists of 453 amino acids and predicts a molecular mass of 50.80 kDa. It migrates as an approximately 65.58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD244 CD244 molecule, natural killer cell receptor 2B4 [ Homo sapiens ]
• Official Symbol: CD244
• Synonyms: CD244; CD244 molecule, natural killer cell receptor 2B4; CD244 natural killer cell receptor 2B4 , natural killer cell receptor 2B4; natural killer cell receptor 2B4; 2B4; NAIL; NKR2B4; Nmrk; SLAMF4; h2B4; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NK cell activation inducing ligand NAIL
• Gene ID: 51744
• mRNA Refseq: NM_001166663
• Protein Refseq: NP_001160135
• MIM: 605554
• UniProt ID: Q9BZW8