Active Recombinant Human CD19 protein, mFc-tagged

• Cat.No.: CD19-3069H
• Product Overview: Recombinant Human CD19 protein(Pro 20-Lys 291), fused with mFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human CD19 (20-291), Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse / Human chimeric FMC63 MAb (human IgG1) with a linear range of 0.5-8 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 57.0 kDa. The protein migrates as 75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Pro 20-Lys 291
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391