Active Recombinant Human CAPZA1/CAPZB Protein, His-tagged

• Cat.No.: CAPZA1&CAPZB-869H
• Product Overview: Recombinant Human CAPZA1&CAPZB(non-muscle isoform) with the alpha-1 SU untagged and beta-2 SU His-tagged was produced in HEK293 cells.

Specification

• Description: The subunits of the heterodimeric capping protein CapZ possess a molecular mass of 34kDa (alpha 1-subunit) and 32kDa (beta 2-subunit, His-tagged). CapZ binds with high affinity in a Ca++-independent manner to the barbed end of actin filaments (1:1000 M/M). Ca++-insensitive barbed end capping protein. Kd actin binding = >1nM (barbed end) .
• Source: HEK293
• Species: Human
• Tag: His
• Form: 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1.0ml ultrapure water to obtain a 1.0 mg/ml solution.
• Bio-activity: Capping activity: F-actin containing 20% pyrene actin was incubated with CapZ and diluted below the critical concentration. Depolymerisation was followed by fluorometry and compared to an actin reference without CapZ.
  Complete capping was obtained at a molar ratio of 1 : 1000 (CapZ : Actin). All proteins were used as supplied, i.e. without dialysis. G-Actin stock solutions were pre-spun 1h, 100.000xg, 4 centigrade.
• Molecular Mass: 66kDa
• Purity: >94% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
• Storage: CapZ is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.
• Shipping: At ambient temperature. Upon delivery store at -70 centigrade.

Gene Information

• Gene Name: CAPZA1 capping protein (actin filament) muscle Z-line, alpha 1 [ Homo sapiens ]
• Official Symbol: CAPZA1
• Synonyms: CAPZA1; capping protein (actin filament) muscle Z-line, alpha 1; F-actin-capping protein subunit alpha-1; Cap Z; capZ alpha-1; CAPZ; CAZ1; CAPPA1
• Gene ID: 829
• mRNA Refseq: NM_006135
• Protein Refseq: NP_006126
• MIM: 601580
• UniProt ID: P52907