Active Recombinant Human BTLA Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: BTLA-566H
• Product Overview: Biotinylated Recombinant Human BTLA Protein (Lys 31 - Thr 134) (Q7Z6A9), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: Lys 31 - Thr 134
• Predicted N Terminal: Lys 31
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human HVEM, Fc Tag at 10μg/mL (100 μL/well) can bind Biotinylated Human BTLA, Fc Tag with a linear range of 0.3-5 μg/mL.
• Molecular Mass: Recombinant Human BTLA Protein has a calculated MW of 40.5 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9
• Chromosome Location: 3q13.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem
• Function: receptor activity