Active Recombinant Human BTK protein, His-tagged

• Cat.No.: BTK-398H
• Product Overview: Recombinant Human BTK protein(NP_000052.1)(Met 1-Ser 659), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: Met 1-Ser 659
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be >50 nmol/min/mg using Poly(Glu,Tyr)4:1 peptide as substrate.
• Molecular Mass: The secreted recombinant human BTK consists of 670 amino acids and predicts a molecular mass of 77.8 KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AGMX1, IMD1; tyrosine-protein kinase BTK; ATK; PSCTK1; XLA; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase; AT; BPK; IMD1; AGMX1; MGC126261; MGC126262
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187