Active Recombinant Human BMP7 Protein (Met315-His431), C-His tagged, Animal-free, Carrier-free

• Cat.No.: BMP7-282H
• Product Overview: Recombinant Human BMP7 Protein (Met315-His431) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone Morphogenetic Protein-7 (BMP-7) is an extracellular multifunctional cytokine that is also a member of the TGF-β family. BMP7 can significantly inhibit TGF-β through binding with TGF-β receptor and trigger SMAD transcription factors, such as SMAD 1 /5/8. It plays a vital role in inhibiting the expansion of kidney damage and promoting the differentiation of osteoblasts.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 0.65 μg/mL.
• Molecular Mass: The protein has a calculated MW of 14.00 kDa.
  The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Met315-His431
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: BMP7 bone morphogenetic protein 7 [ Homo sapiens (human) ]
• Official Symbol: BMP7
• Synonyms: BMP7; bone morphogenetic protein 7; OP 1; osteogenic protein 1; BMP-7; OP-1
• Gene ID: 655
• mRNA Refseq: NM_001719
• Protein Refseq: NP_001710
• MIM: 112267
• UniProt ID: P18075