Active Recombinant Human BMP4 Protein, Pre-aliquoted

• Cat.No.: BMP4-11H
• Product Overview: Recombinant Human BMP4 Protein, Ala18 - Glu144, was expressed in E. coli.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/mL.
• Molecular Mass: Predicted Molecular Mass:14 kDa
  SDS-PAGE: 14 kDa, reducing conditions
• Protein length: Ala18-Glu144
• N-terminal Sequence Analysis: Ala18
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in PBS containing ≥ 0.1% human or bovine serum albumin, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: BMP4 bone morphogenetic protein 4 [ Homo sapiens (human) ]
• Official Symbol: BMP4
• Synonyms: BMP4; bone morphogenetic protein 4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6; bone morphogenetic protein 4; bone morphogenetic protein 2B
• Gene ID: 652
• mRNA Refseq: NM_001202
• Protein Refseq: NP_001193
• MIM: 112262
• UniProt ID: P12644