Active Recombinant Human BMP3 Protein (Gln363-Arg472), C-His tagged, Animal-free, Carrier-free

• Cat.No.: BMP3-278H
• Product Overview: Recombinant Human BMP3 Protein (Gln363-Arg472) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Gln363-Arg472
• Description: Bone Morphogenetic Protein-3 (BMP-3) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-3 can bind with TGF-β receptor and participate in SMAD protein signal transduction through triggering pathway-restricted SMAD protein phosphorylation. The primary function of BMP-3 is osteoblast differentiation and Induces bone formation.
• Form: Lyophilized
• Bio-activity: Measure by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 10 μg/mL.
• Molecular Mass: The protein has a calculated MW of 13.34 kDa.
  The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: BMP3 bone morphogenetic protein 3 [ Homo sapiens (human) ]
• Official Symbol: BMP3
• Synonyms: BMP3; bone morphogenetic protein 3; bone morphogenetic protein 3 (osteogenic); osteogenin; BMP-3; bone morphogenetic protein-3; bone morphogenetic protein 3A; BMP-3A
• Gene ID: 651
• mRNA Refseq: NM_001201
• Protein Refseq: NP_001192
• MIM: 112263
• UniProt ID: P12645