Active Recombinant Human BMP2

• Cat.No.: BMP2-23H
• Product Overview: Recombinant, human bone morphogenetic protein-2(BMP-2) expressed in CHO cell and purified byproprietary chromatographic methods.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: BMP-2 is a homodimeric protein that belongs to the transforming growth factor β (TGF-β) superfamily. BMP-2 is known to regulate diverse fundamental processes during embryonic development. BMP-2 have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells.
• Form: Lyophilized from a 0.2 μm filtered solution in 0.2% sucrose, 2.5% Glycine, 0.01% polysorbate 80, 0.01%sodium chloride, 3% mannitol per 1 μg of BMP-2.
• Bio-activity: The ED50 as determined by cytolysis of ATDC-5 cell is less than 50 ng/ml.
• Molecular Mass: The molecule has a calculated molecular mass of approximately 26 kDa. Due to glycosylation, rhBMP-2migrates as an approximately 36 kDa protein under non-reducing conditions and as a 17 - 18 kDa proteinunder reducing conditions in SDS-PAGE.
• Endotoxin: <0.5 eu per 1 μg of bmp-2 as determined by the lal</0.5
• Purity: >95% as determined by SEC-HPLC and SDS-PAGE.
• Storage: Lyophilized samples are stable for up to six months at -20° C to -70° C.
• Reconstitution: Reconstitute to 100 μg/mL with sterile distilled water. Further dilutions can be made in aqueous buffersjust prior to use.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643
• Chromosome Location: 20p12
• Pathway: Adipogenesis, organism-specific biosystem; BMP Signalling Pathway, organism-specific biosystem; Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Heart Development, organism-specific biosystem
• Function: BMP receptor binding; SMAD binding; activin receptor activity, type II; cytokine activity; growth factor activity; phosphatase activator activity; protein binding; protein domain specific binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; retinol dehydrogenase activity; transmembrane receptor protein serine/threonine kinase activity