Active Recombinant Human BLAME protein, His-Avi-tagged, Biotinylated

• Cat.No.: BLAME-2937H
• Product Overview: Biotinylated Recombinant Human BLAME protein(Ala 23-Asp 233), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Ala 23-Asp 233
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Citrate, 150 mM NaCl, pH5.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human BLAME, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BLAME, His,Avitag with a linear range of 1-31 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 27.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.