Active Recombinant Human BDNF Protein

• Cat.No.: BDNF-143H
• Product Overview: Recombinant Human Brain-Derived Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding His129-Arg247 is expressed.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 129-247 a.a.
• Description: Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family. Along with other structurally related neurotrophic factors NGF, NT-3 and NT-4, BDNF binds with high affinity to the TrkB kinase receptor. It also binds with the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). BDNF promotes the survival, growth and differentiation of neurons. It serves as a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. BDNF expression is altered in neurodegenerative disorders such as Parkinson's and Alzheimer's disease.
• Form: Lyophilized from a 0.2 um filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Bio-activity: Immobilized Human BDNF at 2ug/mll (100 μl/well) can bind Human TrkB-His. The ED50 of Human BDNF is 2-10 ug/ml.
• Molecular Mass: Predicted Molecular Weight: 13kDa
  Apparent Molecular Weight: 14kDa, reducing conditions.
• AA Sequence: His129-Arg247
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Quality Statement: Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: BDNF brain derived neurotrophic factor [ Homo sapiens (human) ]
• Official Symbol: BDNF
• Synonyms: Brain-Derived Neurotrophic Factor; Abrineurin; ANON2; BULN2
• Gene ID: 627
• mRNA Refseq: NM_001143805.1
• Protein Refseq: NP_001137277.1
• MIM: 113505
• UniProt ID: P23560