Active Recombinant Human BACE1 protein(Met1-Thr457), hFc-tagged

• Cat.No.: BACE1-253H
• Product Overview: Recombinant Human BACE1 protein(NP_036236.1)(Met1-Thr457), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Thr457
• similar: BACE1
• Alias: BACE1; beta-site APP-cleaving enzyme 1; BACE, beta site APP cleaving enzyme; beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme; ASP2; BACE; HSPC104; FLJ90568; KIAA1149
• Conjugate: C-hFc
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bioactivity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >0.5 pmoles/min/μg.
• MolecularMass: The recombinant human BACE1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 674 amino acids and has a calculated molecular mass of 75 kDa. As a result of glycosylation, the rhBACE1/Fc monomer migrates as an approximately 100-105 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneName: BACE1 beta-site APP-cleaving enzyme 1 [ Homo sapiens ]
• GeneNameWeblink: 23621
• OfficialSymbol: BACE1
• OfficialSymbolWeblink: 23621
• GeneID: 23621
• GeneIDWeblink: 23621
• mRNARefseq: NM_001207048
• mRNARefseqWeblink: NM_001207048.1
• ProteinRefseqWeblink: NP_001193977.1
• MIMWeblink: 604252
• UniProtID: P56817
• UniProtIDWeblink: P56817

Gene Information

• Synonyms: BACE1; beta-site APP-cleaving enzyme 1; BACE, beta site APP cleaving enzyme; beta-secretase 1; asp 2; memapsin-2; APP beta-secretase; aspartyl protease 2; beta-site APP cleaving enzyme 1; beta-secretase 1 precursor variant 1; transmembrane aspartic proteinase Asp2; membrane-associated aspartic protease 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme; ASP2; BACE; HSPC104; FLJ90568; KIAA1149
• MIM: 604252