Active Recombinant Human AZGP1, Met&His-tagged

• Cat.No.: AZGP1-297H
• Product Overview: Recombinant Human AZGP1 (Accession # AAH05346) Glu2-Glu222, fused with an N-terminal Met and 6-His tag, was produced in E. coli-derived.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-222 a.a.
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit carboxypeptidase A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The IC50 value is <1 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Human AZGP1, Met&His-tagged has a calculated MW of 27 kDa. In SDS-PAGE migrates as 30 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: AZGP1 alpha-2-glycoprotein 1, zinc-binding [ Homo sapiens ]
• Official Symbol: AZGP1
• Synonyms: AZGP1; alpha-2-glycoprotein 1, zinc-binding; alpha 2 glycoprotein 1, zinc; zinc-alpha-2-glycoprotein; ZA2G; ZAG; zn-alpha-2-GP; Zn-alpha2-glycoprotein; zn-alpha-2-glycoprotein; Alpha-2-glycoprotein, zinc
• Gene ID: 563
• mRNA Refseq: NM_001185
• Protein Refseq: NP_001176
• MIM: 194460
• UniProt ID: P25311
• Chromosome Location: 7q22.1